GABA-receptor complex in monkeys treated with MPTP

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5318888

Huffman, R D; Ticku, M K

Tissue samples from the brains of monkeys made parkinsonian by the depletion of dopamine (DA) with dopaminergic neurotoxin (N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (1.4-3.4 mg/kg, i.v.) were assayed for changed in GABA ((/sup 3/H)-GABA), benzodiazepine ((/sup 3/H)-flunitrazepam) and picrotoxin ((/sup 35/S)-TBPS) binding sites. One point binding assays were performed on globus pallidus (GP), substantia nigra reticulata (SN/sub R/) and VA-VL thalamic samples. GABA binding was markedly increased in the SN/sub R/ (129 +/- 12%, n = 2) and GP (108 +/- 33%, n = 4) and not altered in the striatum or thalamus. However, benzodiazepine binding was increased in the striatum (170%; 257 fm/mg, control; 692 fm/mg, treated) and GP (28%; 317 fm/mg, control, 405 fm/mg, treated) and (/sup 35/S)-TBPS binding was also increased in GP (100%; 32.5 fm/mg, control; 65.5 fm/mg, treated). atScatchard analysis of (/sup 3/H)-GABA binding was also performed on tissue samples of motor cortex, cerebellar vermis and striatum pooled from half brains of 4 parkinsonian and 2 control monkeys. Depletion of DA (92 +/- 5%) in the striatum of these monkeys was not associated with any change in the K/sub D/ or B/sub max/ for the high or low affinity GABA binding sites in the striatum, motor cortex or cerebellum. Thus, in the basal ganglia, DA depletion is associated with an increase in GABA binding sites in GP and SN/sub R/, an increase in picrotoxin binding sites in GP and an increase in benzodiazepine binding sites in the striatum.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 5318888
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DAYS LIVING RADIOISOTOPES
DISEASES
DOPAMINE
DRUGS
EVEN-ODD NUCLEI
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MONKEYS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NEUROREGULATORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
PSYCHOTROPIC DRUGS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SULFUR 35
SULFUR ISOTOPES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRANQUILIZERS
TRITIUM COMPOUNDS
VERTEBRATES