Inhibition of. beta. -bungarotoxin binding to brain membranes by mast cell degranulating peptide, toxin I, and ethylene glycol bis(. beta. -aminoethyl ether)-N,N,N',N'-tetraacetic acid

Journal Article · Tue Feb 09 00:00:00 EST 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00403a019· OSTI ID:5317169

Schmidt, R R; Betz, H; Rehm, H

The presynaptically active snake venom neurotoxin ..beta..-bungarotoxin (..beta..-Butx) is known to affect neurotransmitter release by binding to a subtype of voltage-activated K/sup +/ channels. Here the authors show that mast cell degranulating (MCD) peptide from bee venom inhibits the binding of /sup 125/I-labeled ..beta..-Butx to chick and rat brain membranes with apparent K/sub i/ values of 180 nM and 1100 nM, respectively. The mechanisms of inhibition of MCD peptide is noncompetitive, as is inhibition of /sup 125/I-..beta..-Butx binding by the protease inhibitor homologue from mamba venom, toxin I. ..beta..-Butx and its binding antagonists thus bind to different sites of the same membrane protein. Removal of Ca/sup 2 +/ by ethylene glycol bis(..beta..-aminoethyl ether)-N,N,N',N'-tetraacetic acid inhibits the binding of /sup 125/I-..beta..-Butx by lowering its affinity to brain membranes.

• Research Organization: Universitaet Heidelberg (West Germany)
• OSTI ID: 5317169
• Journal Information: Biochemistry; (United States), Vol. 27:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
BIOCHEMICAL REACTION KINETICS
CONFIGURATION INTERACTION
LABELLED COMPOUNDS
TOXINS
BRAIN
CHICKENS
CYTOCHROMES
GLYCOLS
INHIBITION
IODINE 125
MAST CELLS
PEPTIDE HYDROLASES
PEPTIDES
RATS
VENOMS
ALCOHOLS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIRDS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FOWL
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PIGMENTS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques