Effects of the differentiating agents sodium butyrate and N-methylformamide on the oxygen enhancement ratio of human colon tumor cells
Journal Article
·
· Radiat. Res.; (United States)
We have previously shown that chronic adaptation of human tumor cells to the differentiation-inducing agents N-methylformamide (NMF) and sodium butyrate (NAB) increases the sensitivity of oxic cells to graded single doses of X rays. These studies were carried out to define the sensitivity of hypoxic cells after adaptation. Clone A colon tumor cells were grown for three passages in medium containing 170 mM NMF or 2 mM NAB and irradiated in suspension culture, after gassing with either oxygen (60 min) or ultrapure nitrogen (90 min), and complete survival curves were generated. Using the linear-quadratic equation to describe the data, it was found that NMF and NAB produced increased X-ray killing of hypoxic cells. At the 10% level of survival, the dose-modifying factors were about 1.20 and 1.25 for NMF- and NAB-adapted hypoxic cells, respectively, as compared to hypoxic control cells. However, since both oxic and hypoxic cells exhibited increased sensitivity after NMF and NAB adaptation, there was no major change in the oxygen enhancement ratio.
- Research Organization:
- Rhode Island Hospital, Providence (USA)
- OSTI ID:
- 5314194
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 113:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enhanced X ray sensitivity of human colon tumor cells by combination of N-methylformamide with chemotherapeutic agents
X ray responses of a human colon tumor cell line after exposure to the differentiation-inducing agent N-methylformamide: concentration dependence and reversibility characteristics
Changes in X-ray sensitivity and glutathione content of human colon tumor cells after exposure to the differentiation-inducing agent sodium butyrate
Journal Article
·
Fri Aug 01 00:00:00 EDT 1986
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
·
OSTI ID:6914813
X ray responses of a human colon tumor cell line after exposure to the differentiation-inducing agent N-methylformamide: concentration dependence and reversibility characteristics
Journal Article
·
Wed Jun 01 00:00:00 EDT 1988
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
·
OSTI ID:6908300
Changes in X-ray sensitivity and glutathione content of human colon tumor cells after exposure to the differentiation-inducing agent sodium butyrate
Journal Article
·
Wed Jun 01 00:00:00 EDT 1988
· Radiat. Res.; (United States)
·
OSTI ID:6971625
Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
BODY
CELL CULTURES
CELL KILLING
DIGESTIVE SYSTEM
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
EQUATIONS
GASTROINTESTINAL TRACT
INTESTINES
IONIZING RADIATIONS
LARGE INTESTINE
MAMMALS
MAN
NONMETALS
ORGANS
OXYGEN
PRIMATES
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
BODY
CELL CULTURES
CELL KILLING
DIGESTIVE SYSTEM
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
EQUATIONS
GASTROINTESTINAL TRACT
INTESTINES
IONIZING RADIATIONS
LARGE INTESTINE
MAMMALS
MAN
NONMETALS
ORGANS
OXYGEN
PRIMATES
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
X RADIATION