Interaction of urokinase A chain with the receptor of human keratinocytes stimulates release of urokinase-like plasminogen activator

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(90)90112-N· OSTI ID:5311934

Fibbi, G; Magnelli, L; Pucci, M; Del Rosso, M ^[1]

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1. Florence Univ. (Italy)

On the basis of a fibrinolytic assay with {sup 125}I-fibrin, zymography, and immunoprobing with anti-human urokinase antibody, the authors have observed that the in vitro established NCTC human keratinocyte cell line releases into the culture medium a 54,000-Da plasminogen activator which is indistinguishable from human urokinase. Only the early release following the washing of keratinocyte monolayers is accounted for by secretion of preformed enzyme, while late secretory events require the de novo synthesis of urokinase. The released enzyme can interact by autocriny with its own receptor present on keratinocytes. The addition to the keratinocyte culture medium of the urokinase A chain can stimulate a concentration-dependent urokinase oversecretion, which is not paralleled by oversecretion of plasminogen activator inhibitor-1. Since stimulation of urokinase production can be obtained by an A chain concentration which was previously shown to be efficient in inducing keratinocyte mobilization in an in vitro migration model system, they hypothesize that this mechanism may be important in vivo during the process of wound repair.

• OSTI ID: 5311934
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 187:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BLOOD COAGULATION FACTORS
BODY
CHEMICAL ACTIVATION
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EPIDERMIS
EPITHELIUM
FIBRIN
FIBRINOLYTIC AGENTS
HALIDES
HALOGEN COMPOUNDS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INJURIES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANE PROTEINS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PHOSPHORS
PLASMINOGEN
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
REPAIR
SCLEROPROTEINS
SKIN
SODIUM COMPOUNDS
SODIUM IODIDES
SYNTHESIS
TISSUES
UROKINASE
VERTEBRATES
WOUNDS