Effect of interleukin-1. alpha. on striatal prostaglandin E2, cyclic AMP, and dopamine release in irradiated and nonirradiated rats

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5309692

Kandasamy, S B; Chen, H T; Blakley, S; Dalton, T K; Harris, A H ^[1]

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1. Armed Forces Radiobiology Research Inst., Bethesda, MD (United States)

The purpose of this study was to determine the effect of pretreatment with IL-1{alpha} on irradiated and nonirradiated rats striatal prostaglandin E2 (PGE2) and cyclic AMP (cAMP) levels and in vitro release of dopamine (DA) stimulated by KCl. Rats were irradiated using a LINAC. Striatal PGE2 and cAMP were estimated by radioimmunoassay, and DA was measured by HPLC coupled to electrochemical detection. A 20-hr pretreatment with 10-50 {mu}/kg IP of IL-1{alpha} increased striatal PGE2, had no significant effect on cAMP, but enhanced 30 mM KCl-stimulated DA release in irradiated and nonirradiated animals, although there was no significant difference between them. However, 1-hr pretreatment with IL-1{alpha} had no significant effect on PGE2 and cAMP levels and DA release. IL-1{alpha} enhanced PGE2, and DA releases were attenuated by 1-3 mg/kg IP of indomethacin, a cyclooxygenase inhibitor. Exogenous administration of 500 mM to 5 {mu}M of PGE2 increased 30 mM KCl-stimulated DA release in irradiated and nonirradiated animals. These results suggest that IL-1{alpha} increased KCl-stimulated DA release in striatum is mediated by PGE2 because IL-1{alpha} increased PGE2 levels in vivo and exogenous administration of PGE2 enhanced DA release.

• OSTI ID: 5309692
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMP
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOASSAY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
DOPAMINE
DRUGS
GROWTH FACTORS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOKINES
MAMMALS
MITOGENS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SECRETION
SEPARATION PROCESSES
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES