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Purification and characterization of a Saccharomyces cerevisiae exoribonuclease which yields 5'-mononucleotides by a 5'. -->. 3' mode of hydrolysis

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:5308498
An exoribonuclease producing 5'-mononucleotides has been purified from ribosomes of Saccharomyces cerevisiae. The enzyme has a broad pH optimum around 8.0, requires divalent cation, and is stimulated by monovalent cation with the cation and degree of stimulation being dependent on the substrate used. With either poly(a) or rRNA as substrate, the enzyme has a processive mode of hydrolysis. The oligonucleotides, (pA)/sub 3-5/, are hydrolyzed by the enzyme, and the hydrolysis is dependent on a 5'-phosphate end group. Phosphorylation of the 3' end has little effect on the rate of hydrolysis. With (/sup 3/H)poly(A) or (/sup 3/H)rRNA, labeled differentially at the 5' termini, a more rapid release of 5'-terminal label can be shown, providing evidence that the enzyme hydrolyzes in a 5' ..-->.. 3' direction. Further evidence for a 5' ..-->.. 3' mode of hydrolysis is provided by a study of the products of the hydrolysis of (/sup 3/H)(pA)/sub 5/ labeled at the 5' termini with /sup 32/P. No /sup 32/P label is found in (pA)/sub 2/ which accumulates as an intermediate.
Research Organization:
Oak Ridge National Lab., TN
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
5308498
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 255:7; ISSN JBCHA
Country of Publication:
United States
Language:
English

Similar Records

Evidence for a 5'. -->. 3' direction of hydrolysis by a 5' mononucleotide-producing exoribonuclease from Saccharomyces cerevisiae
Journal Article · Sun Dec 31 23:00:00 EST 1978 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:5127304
Exoribonuclease from Saccharomyces cerevisiae: effect of modifications of 5' end groups on the hydrolysis of substrates to 5' mononucleotides
Journal Article · Wed Mar 29 23:00:00 EST 1978 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:6550273
Exoribonuclease from Saccharomyces cerevisiae: effect of modifications of 5' end groups on the hydrolysis of substrates to 5' mononucleotides
Journal Article · Wed Mar 29 23:00:00 EST 1978 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:6850984

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
FUNGI
HYDROGEN ISOTOPES
HYDROLYSIS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MESSENGER-RNA
METABOLITES
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PLANTS
PURIFICATION
RADIOISOTOPES
REACTION KINETICS
RIBOSOMES
RNA
RNA-ASE
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SEPARATION PROCESSES
SOLVOLYSIS
TRANSFERASES
TRITIUM
YEARS LIVING RADIOISOTOPES
YEASTS