Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media
Journal Article
·
· Am. Rev. Respir. Dis.; (United States)
OSTI ID:5307781
The formulation of media for selective, automatable, radiometric detection of growth of Mycobacterium tuberculosis in vitro is described. Palmitic-1 acid labeled with carbon-14 and formic-/sup 14/C acid were compared as substrate sources of (/sup 14/C)O2 in media deficient in carbohydrate and containing appropriate antimicrobial agents that are not active against tubercle bacilli. A preliminary clinical laboratory study of a medium containing 4 microCi palmitic-1-/sup 14/C acid per ml showed that this method might provide the basis for practical laboratory use.
- OSTI ID:
- 5307781
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Vol. 115:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
GROWTH
MEASURING METHODS
MYCOBACTERIUM TUBERCULOSIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CULTURE MEDIA
FORMIC ACID
HEXADECANOIC ACID
IN VITRO
BACTERIA
CARBOXYLIC ACIDS
LABELLED COMPOUNDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MYCOBACTERIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
550701* - Microbiology- Tracer Techniques
550301 - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
GROWTH
MEASURING METHODS
MYCOBACTERIUM TUBERCULOSIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CULTURE MEDIA
FORMIC ACID
HEXADECANOIC ACID
IN VITRO
BACTERIA
CARBOXYLIC ACIDS
LABELLED COMPOUNDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MYCOBACTERIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
550701* - Microbiology- Tracer Techniques
550301 - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques