Genomic structure and chromosomal localization of GML (GPI-anchored molecule-like protein), a gene induced by p53

Journal Article · Thu May 01 04:00:00 EDT 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1997.4680· OSTI ID:530748

Kimura, Yasutoshi ^[1]; Furuhata, Tomohisa; Nakamura, Yusuke ^[2]

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1. Univ. of Tokyo (Japan)
2. Univ. of Tokyo (Japan); and others

Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes. We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildtype p53. To characterize the GML gene further, we determined 35.8 kb of DNA sequence that included a consensus binding sequence for p53 and the entire GML gene. The GML gene consists of four exons, and the p53-binding sequence is present in the 5{prime}-flanking region. In genomic organization this gene resembles genes encoding murine Ly-6 glycoproteins, a human homologue of the Ly-6 family called RIG-E, and CD59; products of these genes, known as GPI-anchored proteins, are variously involved in signal transduction, cell-cell adhesion, and cell-matrix attachment. FISH analysis revealed that the GML gene is located on human chromosome 8q24.3. Genes encoding at least two other GPI-anchored molecules, E48 and RIG-E, are also located in this region. 20 refs., 2 figs., 1 tab.

• OSTI ID: 530748
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 41; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
COSMIDS
DNA SEQUENCING
EXONS
FLUORESCENCE
GENE MUTATIONS
GENE REGULATION
GENES
GENETIC MAPPING
GLYCOPROTEINS
HUMAN CHROMOSOME 8
IN-SITU HYBRIDIZATION
POLYMERASE CHAIN REACTION
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION