An integrated genetic and physical map of the autosomal recessive polycystic kidney disease region
- Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States); and others
Autosomal recessive polycystic kidney disease is one of the most common hereditary renal cystic diseases in children. Genetic studies have recently assigned the only known locus for this disorder, PKHD1, to chromosome 6p21-p12. We have generated a YAC contig that spans {approximately}5 cM of this region, defined by the markers D6S1253-D6S295, and have mapped 43 sequence-tagged sites (STS) within this interval. This set includes 20 novel STSs, which define 12 unique positions in the region, and three ESTs. A minimal set of two YACs spans the segment D6S465-D6S466, which contains PKHD1, and estimates of their sizes based on information in public databases suggest that the size of the critical region is <3.1 Mb. Twenty-eight STSs map to this interval, giving an average STS density of <1/150 kb. These resources will be useful for establishing a complete trancription map of the PKHD1 region. 10 refs., 1 fig., 1 tab.
- OSTI ID:
- 530745
- Journal Information:
- Genomics, Vol. 41, Issue 3; Other Information: PBD: 1 May 1997
- Country of Publication:
- United States
- Language:
- English
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