Characterization and chromosomal mapping of the human TFG gene involved in thyroid carcinoma
- Univ. Hospital of Lund (Sweden); and others
Homology searches in the Expressed Sequence Tag Database were performed using SPYGQ-rich regions as query sequences to find genes encoding protein regions similar to the N-terminal parts of the sarcoma-associated EWS and FUS proteins. Clone 22911 (T74973), encoding a SPYGQ-rich region in its 5{prime} end, and several other clones that overlapped 22911 were selected. The combined data made it possible to assemble a full-length cDNA sequence. This cDNA sequence is 1677 bp, containing an initiation codon ATG, an open reading frame of 400 amino acids, a poly(A) signal, and a poly(A) tail. We found 100% identity between the 5{prime} part of the consensus sequence and the 598-bp-long sequence named TFG. The TFG sequence is fused to the 3{prime} end of NTRK1, generating the TRK-T3 fusion transcript found in papillary thyroid carcinoma. The cDNA therefore represents the full-length transcript of the TFG gene. TFG was localized to 3q11-q12 by fluorescence in situ hybridization. The 3{prime} and the 5{prime} ends of the TFG cDNA probe hybridized to a 2.2-kb band on Northern blot filters in all tissues examined. 28 refs., 5 figs., 1 tab.
- OSTI ID:
- 530727
- Journal Information:
- Genomics, Vol. 41, Issue 3; Other Information: PBD: 1 May 1997
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
HUMAN CHROMOSOME 3
GENETIC MAPPING
CHROMOSOMAL ABERRATIONS
THYROID
CARCINOMAS
PATIENTS
HEREDITARY DISEASES
GENES
DNA-CLONING
DNA SEQUENCING
GENE REGULATION
TRANSCRIPTION
CHIMERAS
AMINO ACIDS
PROTEINS
FLUORESCENCE
IN-SITU HYBRIDIZATION
PROBES
POLYMERASE CHAIN REACTION
NUCLEOTIDES