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Title: Amperometric biosensor for the determination of phenols using a crude extract of sweet potato

Abstract

An amperometric biosensor for the determination of phenols is proposed using a crude extract of sweet potato (Ipomoea batatas (L.) Lam.) as an enzymatic source of polyphenol oxidase (PPO; tyrosinase; catechol oxidase; EC 1.14.18.1). The biosensor is constructed by the immobilization of sweet potato crude extract with glutaraldehyde and bovine serum albumin onto an oxygen membrane. This biosensor provides a linear response for catechol, pyrogallol, phenol and p-cresol in the concentration ranges of 2.0 x 10{sup -5} -4.3 x 10{sup -4} mol L{sup -1}, 2.0 x 10{sup -5} -4.3 x 10{sup -4} mol L{sup -1}, 2.0 x 10{sup -5} -4.5 x 10{sup -4} mol L{sup -1} and 2.0 x 10{sup -5} -4.5 x 10{sup -4} mol L{sup -1}, respectively. The response time was about 3-5 min for the useful response range, and the lifetime of this electrode was excellent for fifteen days (over 220 determinations for each enzymatic membrane). Application of this biosensor for the determination of phenols in industrial wastewaters is presented.

Authors:
;  [1]
  1. Universidade Federal de Sa Carlos (Brazil)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
530558
Resource Type:
Journal Article
Journal Name:
Analytical Letters
Additional Journal Information:
Journal Volume: 30; Journal Issue: 5; Other Information: PBD: Mar 1997
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; 54 ENVIRONMENTAL SCIENCES; PHENOLS; AMPEROMETRY; WASTE WATER; CHEMICAL ANALYSIS; POTATOES; INDUSTRIAL WASTES

Citation Formats

Cruz Vieira, I da, and Fatibello-Filho, O. Amperometric biosensor for the determination of phenols using a crude extract of sweet potato. United States: N. p., 1997. Web. doi:10.1080/00032719708002304.
Cruz Vieira, I da, & Fatibello-Filho, O. Amperometric biosensor for the determination of phenols using a crude extract of sweet potato. United States. doi:10.1080/00032719708002304.
Cruz Vieira, I da, and Fatibello-Filho, O. Sat . "Amperometric biosensor for the determination of phenols using a crude extract of sweet potato". United States. doi:10.1080/00032719708002304.
@article{osti_530558,
title = {Amperometric biosensor for the determination of phenols using a crude extract of sweet potato},
author = {Cruz Vieira, I da and Fatibello-Filho, O},
abstractNote = {An amperometric biosensor for the determination of phenols is proposed using a crude extract of sweet potato (Ipomoea batatas (L.) Lam.) as an enzymatic source of polyphenol oxidase (PPO; tyrosinase; catechol oxidase; EC 1.14.18.1). The biosensor is constructed by the immobilization of sweet potato crude extract with glutaraldehyde and bovine serum albumin onto an oxygen membrane. This biosensor provides a linear response for catechol, pyrogallol, phenol and p-cresol in the concentration ranges of 2.0 x 10{sup -5} -4.3 x 10{sup -4} mol L{sup -1}, 2.0 x 10{sup -5} -4.3 x 10{sup -4} mol L{sup -1}, 2.0 x 10{sup -5} -4.5 x 10{sup -4} mol L{sup -1} and 2.0 x 10{sup -5} -4.5 x 10{sup -4} mol L{sup -1}, respectively. The response time was about 3-5 min for the useful response range, and the lifetime of this electrode was excellent for fifteen days (over 220 determinations for each enzymatic membrane). Application of this biosensor for the determination of phenols in industrial wastewaters is presented.},
doi = {10.1080/00032719708002304},
journal = {Analytical Letters},
number = 5,
volume = 30,
place = {United States},
year = {1997},
month = {3}
}