5'-structural analysis of genes encoding polymorphic antigens of chemically induced tumors
The authors have proposed that the distinct tumor rejection antigens of chemically induced sarcomas in inbred mice belong to a family of M/sub r/ 96,000 glycoproteins (gp96). An identical 14-amino acid sequence was found at the amino terminus of gp96 from two antigenically distinct BALB/c sarcomas. Oligonucleotide probes, end-labeled with (/sup 12/P)-ATP, derived from this sequence permitted isolation of 5' cDNA and genomic fragments coding for gp96. Three short exons interrupted by relatively long introns were identified at the 5' terminus of the gp96 gene. The first exon encodes a signal peptide, which is consistent with gp96 being a cell surface antigen. Southern blot analysis indicated that the gp96 family is encoded by a single gene, and 3-kilobase transcripts were detected in all normal and tumor cells tested. Nucleotide and deduced amino acid sequences from 311 base pairs at the 5' terminus showed no homology with any know protein. The availability of molecular probes for the gp96 system permits analysis of the structural polymorphism of these antigens.
- Research Organization:
- Memorial Sloan-Kettering Cancer Center, New York NY
- OSTI ID:
- 5302974
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:12
- Country of Publication:
- United States
- Language:
- English
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ANTIGENS
DNA SEQUENCING
LABELLED COMPOUNDS
HYBRIDIZATION
MESSENGER-RNA
ATP
GENES
GLUCOPROTEINS
MICE
NEOPLASMS
OLIGONUCLEOTIDES
PHOSPHORUS 32
SARCOMAS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques