Cell volume regulation in hemoglobin CC and AA erythrocytes

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5302641

Berkowitz, L R; Orringer, E P

Swelling hemoglobin CC erythrocytes stimulates a ouabain-insensitive K flux that restores original cell volume. Studies were performed with the K analog, /sup 86/Rb. This volume regulatory pathway was characterized for its anion dependence, sensitivity to loop diuretics, and requirement for Na. The swelling-induced K flux was eliminated if intracellular chloride was replaced by nitrate and both swelling-activated K influx and efflux were partially inhibited by 1 mM furosemide or bumetanide. K influx in swollen hemoglobin CC cells was not diminished when Na in the incubation medium was replaced with choline, indicating Na independence of the swelling-induced flux. Identical experiments with hemoglobin AA cells also demonstrated a swelling-induced increase in K flux, but the magnitude and duration of this increase were considerably less than that seen with hemoglobin CC cells. The increased K flux in hemoglobin AA cells was likewise sensitive to anion replacement and to loop diuretics and did not require the presence of Na. These data indicate that a volume-activated K pathway with similar transport characteristics exists in both hemoglobin CC and AA red cells.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5302641
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATIONS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
ERYTHROCYTES
GLOBIN
GLYCOSIDES
HALIDES
HALOGEN COMPOUNDS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MATERIALS
MEMBRANE TRANSPORT
MINUTES LIVING RADIOISOTOPES
NITRATES
NITROGEN COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
POTASSIUM COMPOUNDS
PROTEINS
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM CHLORIDES
RUBIDIUM COMPOUNDS
RUBIDIUM ISOTOPES
SODIUM COMPOUNDS
STROPHANTHINS
VOLUME