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Reaction of nucleic acids with cis-diamminedichloroplatinum(II): interstrand cross-links

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00397a005· OSTI ID:5301794
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In the reaction of cis-diamminedichloroplatinum(II) (cis-DDP) with double-helical (dC-dG)/sub 4/-(dC-dG)/sub 4/ or (dC-dG)/sub 5/-(dC-dG)/sub 5/, intrastrand and interstrand cross-links between two guanine residues are formed. This is shown by gel electrophoresis in denaturing conditions of the reaction products and by high-performance liquid chromatography (HPLC) analysis of the products digested with nuclease P1. In the reaction of cis-DDP and poly(dG-dC)-poly(dG-dC), at relatively low levels of platination, it is mainly interstrand cross-links between two guanine residues that are formed. This is shown by HPLC analysis of the nuclease P1 digest and by gel electrophoresis in denaturing and nondenaturing conditions of the platinated polymer after cleavage with the restriction enzyme HhaI. Moreover, the antibodies to platinated poly(dG-dC)-poly(dG-dC) cross-react with the interstrand cross-linked (dC-dG)/sub 4/ or (dC-dG)/sub 5/ but not with the intrastrand cross-linked (dG-dC)/sub 4/ or (dC-dG)/sub 5/. These antibodies cross-react with platinated natural DNA. The amount of interstrand cross-links deduced from radioimmunoassays (0.5% of the total bound platinum) is lower than that (2%) deduced by gel electrophoresis in denaturing conditions of a platinated DNA restriction fragment. By gel electrophoresis, it is also shown that in vitro the isomer trans-DDP is more efficient in forming interstrand cross-links than cis-DDP.
Research Organization:
Centre de Biophysique Moleculaire, Orleans, France
OSTI ID:
5301794
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:23; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ATP
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLATINUM COMPOUNDS
POLYMERIZATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS