A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain. alpha. encodes a polyferredoxin
- Ohio State Univ., Columbus (USA)
The genes mvhDGA, which encode the subunit polypeptides of the methyl viologen-reducing hydrogenase in Methanobacterium thermoautotrophicum strain {Delta}H, have been cloned and sequenced. These genes, together with a fourth open reading frame designated mvhB, are tightly linked and appear to form an operon that is transcribed starting 42 base pairs upstream of mvhD. The organization and sequences of the mvhG and mvhA genes indicate a common evolutionary ancestry with genes encoding the small and large subunits of hydrogenases in eubacterial species. The product of the mvhB gene is predicted to contain six tandomly repeated bacterial-ferredoxin-like domains and, therefore, is predicted to be a polyferredoxin that could contain as many as 48 iron atoms in 12 Fe{sub 4}S{sub 4} clusters.
- OSTI ID:
- 5301676
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:9; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYDROGENASES
DNA SEQUENCING
METHANOGENIC BACTERIA
BIOLOGICAL EVOLUTION
DNA-CLONING
FERREDOXIN
GENE AMPLIFICATION
GENES
IRON COMPOUNDS
BACTERIA
CLONING
DNA HYBRIDIZATION
ENZYMES
HYBRIDIZATION
METALLOPROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENT COMPOUNDS
550200* - Biochemistry