Aluminum fluoride induces phosphatidylinositol turnover, elevation of cytoplasmic free calcium, and phosphorylation of the T cell antigen receptor in murine T cells

Journal Article · Sun Nov 15 04:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5301519

O'Shea, J J; Urdahl, K B; Luong, H T; Chused, T M; Samelson, L E; Klausner, R D

Antigen activation of murine T lymphocytes leads to phosphorylation of three subunits of the murine T cell antigen receptor. Two kinases are activated in this process: protein kinase C which leads to phosphorylation of the ..gamma.. and, to a lesser extent, the epsilon subunits on serine residues and a tyrosine kinase which phosphorylates the p21 subunit. The authors sought to determine whether treatment of these cells with NaF could simulate any of these antigen-induced events. Indeed NaF treatment resulted in breakdown of polyphosphoinositides and production of phosphoinositols. This treatment also resulted in a rise in cytosolic free Ca/sup 2 +/. EGTA failed to block this rise suggesting that NaF liberated intracellular stores of Ca/sup 2 +/. Finally NaF treatment resulted in phosphorylation of the ..gamma.. and epsilon chains of the T cell receptor indistinguishable from the effects of phorbol esters. The NaF effect was potentiated by addition of AlCl/sub 3/ consistent with the view that the active moiety is AlF/sub 4//sup -/. The AlF/sub 4//sup -/-induced phosphorylations were abolished in cells in which protein kinase C was depleted by prior treatment with phorbol myristate acetate. All of these observations are compatible with the interpretation that the AlF/sub 4//sup -/ phosphorylation is mediated by protein kinase C. Antigen and anti-receptor antibody-induced receptor serine phosphorylation and phophatidylinositol turnover are blocked by raising intracellular levels of cyclic adenosin monophosphate. In contrast, AlF/sub 4//sup -/-induced effects were in sensitive to cyclic adenosine monmonophosphate

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5301519
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:10; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
ALUMINIUM COMPOUNDS
ALUMINIUM FLUORIDES
ANIMAL CELLS
ANIMALS
ANTIGENS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CARBOHYDRATES
CARCINOGENS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYME INDUCTION
ENZYMES
ESTERS
FLUORIDES
FLUORINE COMPOUNDS
GENE REGULATION
HALIDES
HALOGEN COMPOUNDS
INOSITOLS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES