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Title: On the presence of prostatic secretion protein in rat seminal fluid

Abstract

The copulating plug collected from the tip of the penis from rats immediately after decapitation contains a protein very similar and probably identical to PSP (prostatic secretion protein); this protein has earlier been purified from rat prostatic cytosol and characterized. The protein present in the copulating plug interacts with (3H)estramustine and binds to the antibody raised against rat PSP. The concentration of the protein in the copulating plug is 400 ng/mg of total protein, when measured using the radioimmunoassay technique developed earlier for measurement of PSP in rat prostate. The (3H)estramustine-protein complex formed in a preparation of the copulating plug has an apparent molecular weight of about 50,000 and a sedimentation coefficient of about 3S when analyzed using sucrose density gradient centrifugation. The complex was retained on Concanavalin-A Sepharose indicating that the protein is a glycoprotein. Binding of the complex was also observed on hydroxylapatite and DEAE-Sephadex columns, from which it was eluted at 0.18 M KCl. Light microscope autoradiograms of rat sperms incubated with 125I-labeled PSP indicated that PSP is bound to all parts of the sperms. A macromolecule interacting with the PSP-antibodies is also present in human seminal fluid but at a concentration considerably lower than in ratmore » seminal fluid. The present study shows that a macromolecule probably identical to prostatic secretion protein is present in the copulating plug from the rat. The biological role of this protein in normal male fertility is discussed.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Department of Medical Nutrition, Karolinska Institutet, Stockholm
OSTI Identifier:
5301351
Resource Type:
Journal Article
Resource Relation:
Journal Name: Prostate (N.Y.); (United States); Journal Volume: 2:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOPROTEINS; CHROMATOGRAPHY; MOLECULAR WEIGHT; IODINE 125; AUTORADIOGRAPHY; SPERMATOZOA; FERTILITY; PROSTATE; RATS; SECRETION; TRITIUM COMPOUNDS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GAMETES; GERM CELLS; GLANDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; MALE GENITALS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RODENTS; SACCHARIDES; SEPARATION PROCESSES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Borgstroem, E., Pousette, A., Bjoerk, P., Hoegberg, B., Carlstroem, K., Sundelin, B., and Gustafsson, J.A. On the presence of prostatic secretion protein in rat seminal fluid. United States: N. p., 1981. Web. doi:10.1002/pros.2990020410.
Borgstroem, E., Pousette, A., Bjoerk, P., Hoegberg, B., Carlstroem, K., Sundelin, B., & Gustafsson, J.A. On the presence of prostatic secretion protein in rat seminal fluid. United States. doi:10.1002/pros.2990020410.
Borgstroem, E., Pousette, A., Bjoerk, P., Hoegberg, B., Carlstroem, K., Sundelin, B., and Gustafsson, J.A. Thu . "On the presence of prostatic secretion protein in rat seminal fluid". United States. doi:10.1002/pros.2990020410.
@article{osti_5301351,
title = {On the presence of prostatic secretion protein in rat seminal fluid},
author = {Borgstroem, E. and Pousette, A. and Bjoerk, P. and Hoegberg, B. and Carlstroem, K. and Sundelin, B. and Gustafsson, J.A.},
abstractNote = {The copulating plug collected from the tip of the penis from rats immediately after decapitation contains a protein very similar and probably identical to PSP (prostatic secretion protein); this protein has earlier been purified from rat prostatic cytosol and characterized. The protein present in the copulating plug interacts with (3H)estramustine and binds to the antibody raised against rat PSP. The concentration of the protein in the copulating plug is 400 ng/mg of total protein, when measured using the radioimmunoassay technique developed earlier for measurement of PSP in rat prostate. The (3H)estramustine-protein complex formed in a preparation of the copulating plug has an apparent molecular weight of about 50,000 and a sedimentation coefficient of about 3S when analyzed using sucrose density gradient centrifugation. The complex was retained on Concanavalin-A Sepharose indicating that the protein is a glycoprotein. Binding of the complex was also observed on hydroxylapatite and DEAE-Sephadex columns, from which it was eluted at 0.18 M KCl. Light microscope autoradiograms of rat sperms incubated with 125I-labeled PSP indicated that PSP is bound to all parts of the sperms. A macromolecule interacting with the PSP-antibodies is also present in human seminal fluid but at a concentration considerably lower than in rat seminal fluid. The present study shows that a macromolecule probably identical to prostatic secretion protein is present in the copulating plug from the rat. The biological role of this protein in normal male fertility is discussed.},
doi = {10.1002/pros.2990020410},
journal = {Prostate (N.Y.); (United States)},
number = ,
volume = 2:4,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}