In vitro cytotoxicity testing of 30 reference chemicals to predict acute human and animal toxicity
- York College, Jamaica (United States)
This study was conducted in cooperation with the Scandinavian Society of Cell Toxicology, as part of the Multicenter Evaluation for In Vitro Cytotoxicity (MEIC), and was designed to develop an in vitro model for predicting acute human and animal toxicity. The technique relies on the ability of cultured transformed rat lung epithelial cells (L2) to incorporate radiolabled amino acids into newly synthesized proteins in the absence or presence of increasing doses of the test chemical, during a 24-hr incubation. IC50 values were extrapolated from the dose-response curves after linear regression analysis. Human toxic blood concentrations estimated from rodent LD50 values suggest that our experimental IC50's are in close correlation with the former. Validation of the data by the MEIC committee shows that our IC50 values predicted human lethal dosage as efficient as rodent LD50's. It is anticipated that this and related procedures may supplement or replace currently used animal protocols for predicting human toxicity.
- OSTI ID:
- 5301297
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Research perspectives for pre-screening alternatives to animal experimentation
A combined approach to investigate the toxicity of an industrial landfill's leachate: Chemical analyses, risk assessment and in vitro assays
Related Subjects
XENOBIOTICS
TOXICITY
AMINO ACIDS
BIOLOGICAL MODELS
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
IN VITRO
LABELLED COMPOUNDS
LUNGS
RATS
REGRESSION ANALYSIS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
ISOTOPE APPLICATIONS
MAMMALS
MATHEMATICS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
STATISTICS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology