Effect of anti-thyroxin autoantibodies on radioimmunoassay of free thyroxin in serum

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5300289

Konishi, J ^[1]; Iida, Y; Kousaka, T; Ikekubo, K; Nakagawa, T; Torizuka, K

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1. Kyoto Univ., Japan

Serum thyroxin was nearly or completely undetectable by radioimmunoassay in an elderly patient with Graves' disease being treated with methimazole. Abnormal binding of thyroxin to antibodies of the IgG variety was shown, the association constant of the complex being 8.0 x 10/sup 8/ L/mol and the binding capacity 6.3 nmol/g of IgG. The effect of the antibody on results of radioimmunoassay of free thyroxin was studied with three commercial kits, two of which (Clinical Assays and Damon Diagnostics) gave essentially the same values as did equilibrium dialysis. The third (Amersham International) gave falsely high results because the /sup 125/I-labeled thyroxin derivative used in the kit was bound by the autoantibody.

• OSTI ID: 5300289
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:6; ISSN CLCHA
• Country of Publication: United States
• Language: English

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