Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.13.4868· OSTI ID:5300110

Ono, Yoshitaka; Fujii, Tomoko; Igarashi, Koichi; Kuno, Takayoshi; Tanaka, Chikako; Kikkawa, Ushio; Nishizuka, Yasutomi ^[1]

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1. Takeda Chemical Industries, Osaka (Japan)

Protein kinase C normally has a tandem repeat of a characteristic cysteine-rich sequence in C{sub 1}, the conserved region of the regulatory domain. These sequences resemble the DNA-binding zinc finger domain. For the {gamma} subspecies of rat brain protein kinase C, various deletion and point mutants in this domain were constructed, and the mutated proteins were expressed in Escherichia coli by using the T7 expression system. Radioactive phorbol 12,13-dibutyrate binding analysis indicated that a cysteine-rich zinc-finger-like sequence was essential for protein kinase C to bind phorbol ester and that one of the two sequences was sufficient for the phorbol ester binding. Conserved region C{sub 2}, another region in the regulatory domain, was apparently needed for the enzyme to require Ca{sup 2+} for phorbol ester binding activity.

• OSTI ID: 5300110
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:13; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
CARBOXYLIC ACIDS
CARCINOGENS
CELL PROLIFERATION
CHEMICAL REACTIONS
CROSS-LINKING
CYSTEINE
DNA
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
ESCHERICHIA COLI
ESTERS
HYDROGEN COMPOUNDS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERIZATION
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
THIOLS
TRANSFERASES
TRITIUM COMPOUNDS
ZINC COMPOUNDS