RNA synthesis in primary cultures of adult rat hepatocytes
The ability of hepatocyte monolayers to synthesize RNA was investigated by measuring (3H)orotic acid incorporation into RNA and the total nuclear RNA polymerase activity as a function of the time in culture. The results demonstrate that primary cultures of hepatocytes maintained in a chemically defined serum- and hormone-free medium are able to synthesize RNA actively. This ability increases within the first 2 d of culture, despite the concomitant decrease in (3H)orotic acid uptake, and decreases only after 3 d. Factors such as serum, insulin, and dexamethasone, known to improve maintenance of functional hepatocytes, markedly stimulate the uptake of labeled precursor without apparently affecting the rate of RNA synthesis by cultured cells. It is suggested that the culture of adult rat hepatocytes provides a useful experimental model for the studies of hormonal regulation of transcription in liver.
- Research Organization:
- Istituto di Fisiologia Generale, Universita di Genova, Italy
- OSTI ID:
- 5299652
- Journal Information:
- In Vitro; (United States), Journal Name: In Vitro; (United States) Vol. 19:4; ISSN ITCSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL CULTURES
CORTICOSTEROIDS
DEXAMETHASONE
DIGESTIVE SYSTEM
GLANDS
GLUCOCORTICOIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OROTIC ACID
PEPTIDE HORMONES
PREGNANES
PYRIMIDINES
RADIOISOTOPES
RATS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM
URACILS
VERTEBRATES
YEARS LIVING RADIOISOTOPES