Sex pheromone receptor proteins. Visualization using a radiolabeled photoaffinity analog
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5299371
A tritium-labeled photoaffinity analog of a moth pheromone was used to covalently modify pheromone-selective binding proteins in the antennal sensillum lymph and sensory dendritic membranes of the male silk moth, Antheraea polyphemus. This analog, (E,Z)-6,11-(/sup 3/H)hexadecadienyl diazoacetate, allowed visualization of a 15-kilodalton soluble protein and a 69-kilodalton membrane protein in fluorescence autoradiograms of electrophoretically separated antennal proteins. Covalent modification of these proteins was specifically reduced when incubation and UV irradiation were conducted in the presence of excess unlabeled pheromone, (E,Z)-6,11-hexadecadienyl acetate. These experiments constitute the first direct evidence for a membrane protein of a chemosensory neuron interacting in a specific fashion with a biologically relevant odorant.
- Research Organization:
- State Univ. of New York, Stony Brook (USA)
- OSTI ID:
- 5299371
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:8; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMALS
ARTHROPODS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
COVALENCE
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
FLUORESCENCE
INSECTS
INVERTEBRATES
KINETICS
LABELLED COMPOUNDS
LEPIDOPTERA
LUMINESCENCE
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR WEIGHT
MOTHS
ORGANIC COMPOUNDS
PHEROMONE
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECEPTORS
SECRETION
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMALS
ARTHROPODS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
COVALENCE
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
FLUORESCENCE
INSECTS
INVERTEBRATES
KINETICS
LABELLED COMPOUNDS
LEPIDOPTERA
LUMINESCENCE
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR WEIGHT
MOTHS
ORGANIC COMPOUNDS
PHEROMONE
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECEPTORS
SECRETION
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION