Phosphorus-31 NMR visibility and characterization of rat liver mitochondrial matrix adenine nucleotides

Journal Article · Tue May 16 04:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00436a030· OSTI ID:5299359

Hutson, S M; Berkich, D; Williams, G D; LaNoue, K F; Briggs, R W ^[1]

---

1. Pennsylvania State Univ., Hershey (USA)

Compartmentation and NMR visibility of mitochondrial adenine nucleotides were quantitated in isolated rat liver mitochondria respiring on succinate and glutamate in vitro at 8 and 25{degree}C. Intra- and extramitochondrial nucleotides were discriminated by adding the chelator trans-1,2-diaminocyclohexane-N,N,N{prime},N{prime}-tetraacetic acid (CDTA). T{sub 1} values of about 0.2-0.3 s for magnesium-bound matrix nucleotides were determined. Adenine nucleotide T{sub 1} values were influenced by the ionic environment; only magnesium-free ATP T{sub 1}'s were affected by temperature. Intra- and extramitochondrial adenine nucleotide ratios were varied in ATP-loaded mitochondria with added ATP and phosphate using the mitochondrial inhibitors oligomycin and carboxyatractyloside, and adenine nucleotides were quantitated by using NMR and enzymatic analysis. There was good agreement between matrix ATP concentrations (magnesium-bound ATP) calculated by using NMR and standard biochemical techniques. Although matrix ADP could be detected by NMR, it was difficult to quantitate accurately by NMR. The data indicate that mitochondrial ATP is NMR-visible in isolated mitochondria in vitro.

• OSTI ID: 5299359
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:10; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADENINES
AMINES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
ATP
AZAARENES
BIOCHEMISTRY
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DICARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DRUGS
GLANDS
GLUTAMIC ACID
HETEROCYCLIC COMPOUNDS
IN VITRO
ISOTOPES
LIGHT NUCLEI
LIVER
MAGNETIC RESONANCE
MAMMALS
MITOCHONDRIA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PURINES
RATS
RESONANCE
RESPIRATION
RODENTS
STABLE ISOTOPES
SUCCINIC ACID
VERTEBRATES