Development of a sensitive radioassay of histamine for in vitro allergy testing

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:5299328

Faraj, B A; Gottieb, G R; Camp, V M; Kutner, M; Lolies, P

A radioenzymatic assay for the measurement of histamine is described, based on the incubation of histamine in the presence of histamine-N-methyl-transferase from rat kidney and (/sup 3/H-methyl)-S-adenosyl-L-methionine (sp act 15 Ci/mmol) in phosphate buffer, 0.05 mole/l, pH 7.9, at 37/sup 0/C for 60 min. The N-(/sup 3/H-methyl)histamine generated was selectively extracted into toluene/isoamyl alcohol (3:2) and the quantity of the tritium in the sample was determined by liquid-scintillation counting. As little as 1 nmol/l of histamine can be detected. The assay is specific, with no cross-reactivity noted for several compounds closely related to histamine. The assay was used to measure the released histamine of a group of allergic subjects following the incubation of their blood with various allergens. A good correlation was found between histamine release from whole blood and the response of skin mast cells to intradermal antigen administration.

• Research Organization: Emory Univ. School of Medicine, Atlanta, GA
• OSTI ID: 5299328
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:1; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALLERGY
AMINES
AZOLES
BODY
ENZYMES
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IN VITRO
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PATIENTS
RADIOASSAY
SKIN
TRANSFERASES
TRITIUM COMPOUNDS