On-plate electrochemical detection for thin-layer chromatography

Journal Article · Tue Jan 31 23:00:00 EST 1989 · Analytical Letters; (USA)

DOI:https://doi.org/10.1080/00032718908052358· OSTI ID:5297945

Brown, G N; Birks, J W; Koval, C A ^[1]

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1. Univ. of Colorado, Boulder (USA)

Voltammetric electrochemical detection (ECD) coupled with tin-layer chromatography (TLC) was demonstrated for the quantification of trace organic compound directly on a TLC plate. The electrochemical detection solvent was a thin layer of aqueous potassium chloride. For undeveloped plates, detection limits for p-anisidine and p-phenetidine were 10 ng and 13 ng, respectively. Linearity was demonstrated over nearly 2 orders of magnitude. After development, detection limits increased by approximately a factor of ten. Advantages of this method over other quantitative TLC methods include sensitivity, speed, simplicity, and cost. In addition, this method is selective for electrochemically active compounds. Major sources of experimental error include spot size reproducibility, working electrode placement, and supporting electrolyte film thickness.

• DOE Contract Number: FG02-84ER13247
• OSTI ID: 5297945
• Journal Information: Analytical Letters; (USA), Journal Name: Analytical Letters; (USA) Vol. 22:2; ISSN ANALB; ISSN 0003-2719
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
400105 -- Separation Procedures
400400 -- Electrochemistry
CHEMICAL ANALYSIS
CHEMISTRY
CHROMATOGRAPHY
DATA
ELECTROCHEMISTRY
ELECTRODES
ELECTROLYTES
EXPERIMENTAL DATA
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACE AMOUNTS
VOLTAMETRY