Structure and interparticle interactions of bovine serum albumin in solution studied by small-angle neutron scattering

Journal Article · Thu Apr 28 04:00:00 EDT 1983 · J. Phys. Chem.; (United States)

DOI:https://doi.org/10.1021/j100232a003· OSTI ID:5296193

Bendedouch, D ^[1]; Chen, S H

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1. Brookhaven National Lab., Upton, NY

A series of small-angle neutron scattering (SANS) measurements were carried out on dilute and moderately concentrated bovine serum albumin (BSA) solutions at two different pH values and at t = 35/sup 0/C. The amount of bound water to the protein was deduced from the zero-contrast point of dilute BSA solutions, in D/sub 2/O and H/sub 2/O solvent mixtures. Detailed analysis of the intensity spectrum from the most dilute BSA solution in D/sub 2/O yields a prolate ellipsoidal shape (a,b,b) of the protein molecule with a = 70 angstrom and b = 20 angstrom. At moderate concentrations, pH 7, with or without salt (LiCl) added, the intensity spectra can be fitted satisfactorily by taking into account both the ellipsoidal shape of the particle and an interparticle interference factor (S(Q)). Calculation of S(Q) assumes a model of equivalent charged hard spheres interacting through a repulsive potential. For moderately concentrated solutions at pH 5.1, S(Q) can be accounted for by introducing an attractive potential between the particles.

• OSTI ID: 5296193
• Journal Information: J. Phys. Chem.; (United States), Journal Name: J. Phys. Chem.; (United States) Vol. 87:9; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ALKALI METAL COMPOUNDS
BARYONS
CHLORIDES
CHLORINE COMPOUNDS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HALIDES
HALOGEN COMPOUNDS
HEAVY WATER
HYDROGEN COMPOUNDS
LITHIUM CHLORIDES
LITHIUM COMPOUNDS
LITHIUM HALIDES
MATHEMATICAL MODELS
MOLECULAR MODELS
NEUTRON SPECTRA
NEUTRONS
NUCLEONS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PH VALUE
PROTEINS
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
WATER