Linkage analysis by two-dimensional DNA typing
- Univ. of Groningen (Netherlands)
- INGENY B.V., Leiden (Netherlands)
- Holland Genetics, Arnhem (Netherlands)
- INGENY B.V., Leiden (Netherlands) Beth Israel Hospital, Boston, MA (United States)
In two-dimensional (2-D) DNA typing, genomic DNA fragments are separated, first according to size by electrophoresis in a neutral polyacrylamide gel and second according to sequence by denaturing gradient gel electrophoresis, followed by hybridization analysis using micro- and minisatellite core probes. The 2-D DNA typing method generates a large amount of information on polymorphic loci per gel. Here, the authors demonstrate the potential usefulness of 2-D DNA typing in an empirical linkage study on the red factor in cattle, and the authors show an example of the 2-D DNA typing analysis of a human pedigree. The power efficiency of 2-D DNA typing in general is compared with that of single-locus typing by simulation. The results indicate that, although 2-D DNA typing is very efficient in generating data on polymorphic loci, its power to detect linkage is lower than single-locus typing, because it is not obvious whether a spot represents the presence of one or two alleles. It is possible to compensate for this lower informativeness by increasing the sample size. Genome scanning by 2-D DNA typing has the potential to be more efficient than current genotyping methods in scoring polymorphic loci. Hence, it could become a method of choice in mapping genetic traits in humans and animals. 13 refs., 5 figs., 4 tabs.
- OSTI ID:
- 5296091
- Journal Information:
- American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 53:6; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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