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Evidence for alterations in luteinizing hormone secreted in rhesus monkeys with normal and inadequate luteal phases using radioreceptor and radioimmunoassay

Journal Article · · Endocrinology; (United States)
A radioreceptor assay (RRA) using porcine granulosa cells and (/sup 125/I)hCG was developed and validated for the measurement of LH or CG. The RRA was used in conjunction with a heterologous RIA employing antiserum against ovine LH and (/sup 125/I)ovine LH (RIA) to measure serum LH in the rhesus monkey throughout the menstrual cycle. Discrepancies were found in the measurement of serum LH using RRA and RIA. Measurements of serum LH using RIA were consistently higher than the measurements of serum LH using RRA in serum from adult intact female and male monkeys and hypophysectomized, ovariectomized, and pregnant monkeys.
Research Organization:
Univ. of Maryland School of Medicine, Baltimore
OSTI ID:
5292366
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 104:5; ISSN ENDOA
Country of Publication:
United States
Language:
English