Assay of mouse-cell clones for retrovirus p30 protein by use of an automated solid-state radioimmunoassay
Journal Article
·
· Virology; (United States)
A solid-state radioimmunoassay system has been developed that is useful for automated analysis of samples in microtiter plates. Assays for interspecies and type-specific antigenic determinants of the C-type retrovirus protein, p30, have been used to identify clones of cells producing this protein. This method allows testing of at least 1000 clones a day, making it useful for studies of frequencies of virus protein induction, defective virus production, and formation of recombinant viruses.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5291105
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 97:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550300 -- Cytology
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AUTOMATION
CELL CULTURES
CLONE CELLS
DESIGN
ISOTOPE APPLICATIONS
MAMMALS
MICE
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RODENTS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES
550300 -- Cytology
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AUTOMATION
CELL CULTURES
CLONE CELLS
DESIGN
ISOTOPE APPLICATIONS
MAMMALS
MICE
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RODENTS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES