Extensive homology of nuclear ribonucleic acid and polysomal poly(adenylic acid) messenger ribonucleic acid between normal and neoplastically transformed cells

Journal Article · Tue Mar 04 04:00:00 EST 1980 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00546a001· OSTI ID:5287628

Moyzis, R K; Grady, D L; Li, D W; Mirvis, S E; Ts'o, P O.P.

A cell line, designated BP6T, derived from Syrian hamster embryo (SHE) cells following treatment with benzo(a)pyrene is capable of producing tumors in newborn hamsters following the injection of as few as 1-10 cells. Polysomal poly(A)mRNA and total nuclear RNA obtained from this highly tumorigenic cell line were compared to RNAs obtained from the nonneoplastic parental embryo cells by a variety of techniques. RNA excess hybridizations to normal cell radiolabeled single-copy DNA or to a single-copy DNA tracer enriched for sequences transcribed in neoplastically transformed cells were unable to detect any significant differences in RNA sequence complexity between normal SHE cells and neoplastic BP6T cells. This finding of extensive homology of polysomal poly(A) mRNA and total nuclear RNA between normal and neoplastic cells, together with our previous finding of extensive homology of the major /sup 35/S-labeled nuclear or cytoplasmic polypeptides observable on two-dimensional gels )Leavitt, J.C., and Moyzis, R.K. (1978) J. Biol. Chem. 253, 2497-2500), demonstrates that the phenotypic changes associated with neoplastic transformation by chemical carcinogens are accompanied by relatively few changes in the qualitative pattern of gene expression in cells cultured in vitro.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 5287628
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 19:5; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
DISEASES
EMBRYOS
HAMSTERS
HYDROCARBONS
MAMMALS
MESSENGER-RNA
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RNA
RODENTS
TOXICITY
VERTEBRATES