Equivalency of endothelial cell growth supplement to irradiated feeder cells in carcinogen-induced morphologic transformation of Syrian hamster embryo cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · JNCI, J. Natl. Cancer Inst.; (United States)

OSTI ID:5282344

Evans, C H; DiPaolo, J A

Endothelial cell growth supplement (ECGS), an extract of bovine neural tissue with growth-promoting properties for human endothelial and epithelial cells and for mouse BALB/c fibroblast-like cells, can be substituted for feeder cells in a quantitative 7-day Syrian hamster embryo cell colony in vitro model of carcinogenesis. Inclusion of 50 or 100 micrograms ECGS/ml medium throughout the 7-day growth period produced results equal to those obtained with feeder cells. The frequency and morphology of normal fibroblast colonies and carcinogen-induced morphologically transformed cell colony growth in the presence of ECGS were similar to those in the presence of feeder cells. A positive dose-response relationship in transformation by benzo(a)pyrene occurred. The frequency of transformed colonies following UV irradiation and treatment of the cells with the cocarcinogenic tumor promoter 12-O-tetradecanoylphorbol 13-acetate was greatly augmented, and lymphotoxin, a lymphokine with anticarcinogenic activity, reduced transformation. Thus ECGS can substitute for feeder cells in supporting in vitro transformation and eliminates a potential complex source of variability for studies where interaction(s) with feeder cells are a consideration. The mechanics of this model system was simplified, and its versatility for the study of physiologic, carcinogenic, and other pathophysiologic processes was broadened.

• Research Organization: National Cancer Institute, Bethesda, Md
• OSTI ID: 5282344
• Journal Information: JNCI, J. Natl. Cancer Inst.; (United States), Journal Name: JNCI, J. Natl. Cancer Inst.; (United States) Vol. 68:1; ISSN JJIND
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL MODELS
CARCINOGENESIS
CFU
CONDENSED AROMATICS
CULTURE MEDIA
DISEASES
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
HAMSTERS
HYDROCARBONS
MAMMALS
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
PATHOGENESIS
PATHOLOGY
RADIATIONS
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES