Effect of pretreatment on the hydrolysis of cellulose by Penicillum funiculosum cellulase and recovery of enzyme

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260250714· OSTI ID:5282331

Rao, M; Seeta, R; Deshpande, V

Penicillium funiculosum produces a complete cellulase which brings about 97% hydrolysis of cotton and has high beta-glucosidase, xylanase, laminarinase, and lichenase activities. This article deals with the effect of different pretreatments on the hydrolysis of sugarcane bagasse by P. funiculosum enzymes and the recovery of enzyme from the insoluble residues. Enzymic saccharification of bagasse pretreated with hot 1N NaOH followed by neutralization with HCl and steam treated under pressure (7 kg/sq cm) gave 63 and 59% saccharification, respectively, in 48 hours. Hemicellulose is not lost in these pretreatments. With a 30% slurry of steam-treated bagasse, a semisolid mass containing 14% sugar was obtained. A 90% recovery of CMCase, beta-glucosidase, and filter paper activity from the hydrolysates was obtained under the following conditions: 1) maintaining the ratio of enzyme to substrate, 2) brief grinding of the residual substrate with glass powder, and 3) addition of 0.4% Tween-80 to the eluting buffer. The high recovery of cellulolytic enzymes indicates that the adsorption of these enzymes on cellulose is not irreversible.

• Research Organization: National Chemical Lab., Poona - 411 008, India Biochemistry Div.
• OSTI ID: 5282331
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 25:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

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AGRICULTURAL WASTES
ALKALINE HYDROLYSIS
BAGASSE
CELLULASE
CHEMICAL REACTIONS
COTTON PLANTS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC WASTES
PENICILLIUM
PLANTS
RECOVERY
SOLVOLYSIS
WASTES