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Differential effects of inhibitors and detergents on the Ca/sup 2 +/-ATPase and Mg/sup 2 +/-ATPase activities of the plasma membrane of a human oat cell carcinoma

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5277133
;

Plasma membranes of human oat cell carcinoma possess Mg/sup 2 +/- and Ca/sup 2 +/-dependent ATPase activities of similar magnitude. These activities exhibit the unusual characteristic of being inactiviated by prolonged incubation of the membrane with 1-2 mM dithiothreitol (DTT). Inactivation by DTT was prevented by lowering the incubation temperature, elevation of the membrane protein concentration, and addition of ATP. Fluorosulfonylbenzoyl adenosine (FSBA), an affinity ATP analog, also inactivates these activities. The Ca/sup 2 +/-ATPase activity appears to be more sensitive to both DTT and FSBA. The Ca/sup 2 +/-ATPase activity is more easily inactivated by Triton X-100, while the Mg/sup 2 +/-ATPase is preferentially activated by digitonin. These differential effects of inhibitors and detergents suggest that the Ca/sup 2 +/-ATPase and Mg/sup 2 +/-ATPase are separate enzymes. Incubation of oat cell carcinoma plasma membrane with (/sup 3/H)FSBA resulted in the labeling of several proteins. A labelled 35,000 dalton protein corresponds to the molecular weight of the oat cell carcinoma plasma membrane Ca/sup 2 +/-ATPase previously purified in this laboratory. The identity of one or more of the other labelled proteins with the Mg/sup 2 +/-ATPase has not been demonstrated, but is presently under investigation.

Research Organization:
Univ. of California at San Diego, La Jolla
OSTI ID:
5277133
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADDITIVES
ADENOSINE
AFFINITY
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ATP
ATP-ASE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CALCIUM COMPOUNDS
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
DETERGENTS
DISEASES
DITHIOLS
EMULSIFIERS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAGNESIUM COMPOUNDS
MAMMALS
MAN
MATERIALS
MEMBRANES
MOLECULAR WEIGHT
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOHYDROLASES
PRIMATES
PROTEINS
REAGENTS
RIBOSIDES
SURFACTANTS
THIOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WETTING AGENTS