Characterization of cell surface antigens reactive with autologous antibodies from human ovarian neoplasms

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5277022

Kutteh, W H

Autologous antibodies eluted from membrane fragments of ovarian epithelial neoplasms have been prepared from cyst and ascites fluids. The predominant membrane-bound immunoglobulin, IgG, was present in a range of 18 to 4275 ng of membrane-bound IgG/ml fluid. The autologous antibodies were strongly reactive with human ovarian neoplastic cell lines and fresh ovarian tumor tissue but not with normal human ovaries, other non-ovarian normal or neoplastic tissue or non-ovarian human cell lines. Human ovarian serous cystadenocarcinoma cell lined number2774 was surface labeled with /sup 125/Iodine using lactoperoxidose. Cells were washed and solubilized with Triton X-100. Membrane antigens were prepared and precipitated with autologous antibodies. Precipitates were washed, electrophoresed on 7.5% polyacrylamide gels and analyzed for radioactivity. Three major bands of activity (molecular weights: 180,000; 160,000 and 120,000) were precipitated with autologous antibodies from two patients with serous cystadenocarcinoma and two patients with papillary adenocarcinoma, but not with normal serum or autologous antibodies from a plural effusion of a patient with colon disease.

• Research Organization: Univ. of Alabama Medical Center, Birmingham
• OSTI ID: 5277022
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
BODY
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
EPITHELIUM
FEMALE GENITALS
GLOBULINS
GONADS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANES
MOLECULAR WEIGHT
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OVARIES
PATIENTS
PRIMATES
PROTEINS
RADIOISOTOPES
TISSUES
VERTEBRATES