Evidence for two modes of DNA degradation in Escherichia coli following ultraviolet irradiation

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574563· OSTI ID:5276148

Fong, K; Bockrath, R C

UV-induced DNA degradation is found in wild-type E. coli strains and is greatly reduced by photoreactivation. UV-induced DNA degradation is not found in an excision repair-deficient strain (uvr A) unless incubated post-UV in the presence of chloramphenicol. Chloramphenicol-enhancible degradation is significant and is not reduced by photoreactivation. Greater DNA degradation is found in cells irradiated frozen at -79/sup 0/C than in cells irradiated at ambient temperature (21/sup 0/C). When the cells are irradiated at -79/sup 0/C the amount of degradation is very slightly decreased by photoreactivation. Chloramphenicol-enhancible DNA degradation does not occur in a lexA strain. These data suggest two modes of UV-induced DNA degradation, one responding to pyrimidine dimers and one responding to lesions other than dimers, with chloramphenicol-enhancible degradation corresponding to the second mode.

• Research Organization: Indiana Univ., Indianapolis
• OSTI ID: 5276148
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBIOTICS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
CHLORAMPHENICOL
DECOMPOSITION
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOREACTIVATION
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION