Methylcobalamin methylation of chloroplatinate: bound chloride, valence state, and relative mutagenicity

Journal Article · Sun Jan 01 04:00:00 EST 1978 · J. Environ. Sci. Health, Part A; (United States)

DOI:https://doi.org/10.1080/10934527809374849· OSTI ID:5274700

Taylor, R T; Happe, J A; Wu, R

In comparison to the closely related complexes K/sub 2/PtC1/sub 4/ and K/sub 2/PtC1/sub 6/, MePtC1/sub 3/ /sup 2-/ is slightly less cytotoxic to auxotrophic Chinese hamster ovary cell line (CHO AUXB1). All three of these chloro-Pt compounds at concentrations of 15 to 70 ..mu..M induce a 6 to 10 fold increase in the frequency of CHO AUXBI revertants. Revertants arising either spontaneously (frequency ca. 5 x 10/sup -7/) or induced with chlorplatinates or 4-nitroquinoline-N-oxide (4-NQO) regain a permanent capacity to grow in medium lacking glycine + adenosine + thymidine. In contrast to the corresponding chloroplatinates, K/sub 2/PdC1/sub 4/ and K/sub 2/PdC1/sub 6/ are not mutagenic in CHO AUXB1 as inducers of reversion to prototrophy. They are also 5 to 10 times less cytotoxic than the chloroplatinates in this CHO AUXB1 system.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 5274700
• Journal Information: J. Environ. Sci. Health, Part A; (United States), Journal Name: J. Environ. Sci. Health, Part A; (United States) Vol. 13:9; ISSN JESED
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOSINE
AMINO ACIDS
ANIMALS
AZINES
CARBOXYLIC ACIDS
CELL CULTURES
CHLORIDES
CHLORINE COMPOUNDS
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
GLYCINE
HALIDES
HALOGEN COMPOUNDS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOLATED VALUES
MAMMALS
MORTALITY
MUTAGENESIS
MUTATION FREQUENCY
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLATINUM CHLORIDES
PLATINUM COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
THYMIDINE
TOLERANCE
TOXICITY
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES