The effect of total-body irradiation on corneal neovascularization in the Fischer 344 rat after chemical cauterization
- Duke Univ. Medical Center, Durham, NC (USA)
Previous investigations of corneal neovascularization after irradiation yielded discordant results. Most studies indicated that new blood vessel formation in the cornea is inhibited by irradiation. However, others reported that angiogenesis after corneal cauterization is similar in both irradiated and nonirradiated animals. To assess the effect of total-body irradiation on neovascularization further, the amount of angiogenesis was determined in irradiated rats after chemically induced corneal injury. Corneal tissue was evaluated quantitatively with computerized image analysis 2, 3, or 4 days postcautery in rats perfused with India ink and gelatin immediately after death. The rats were exposed to a single dose (9 Gy) of total-body irradiation 6 days before corneal cauterization. In both the nonirradiated and irradiated rats, neovascularization increased with the duration of the postcautery interval. The amount of corneal neovascularization was not significantly different in the irradiated and nonirradiated rats at any of the postcautery intervals studied. This investigation suggests that endothelial cell migration plays a more important role than cell replication in the pathogenesis of corneal angiogenesis in the Fischer 344 rat. Moreover, the suppression of corneal angiogenesis by irradiation may be dependent on the experimental conditions and species examined.
- OSTI ID:
- 5270954
- Journal Information:
- Investigative Ophthalmology and Visual Science; (United States), Journal Name: Investigative Ophthalmology and Visual Science; (United States) Vol. 32:7; ISSN 0146-0404; ISSN IOVSD
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
BODY AREAS
CARDIOVASCULAR SYSTEM
CELL DIVISION
CORNEA
DISEASES
ENDOTHELIUM
EXTERNAL IRRADIATION
EYES
FACE
HEAD
IMAGE PROCESSING
IRRADIATION
MAMMALS
ORGANS
PATHOGENESIS
PROCESSING
RADIATION EFFECTS
RATS
RODENTS
SENSE ORGANS
SENSE ORGANS DISEASES
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION