Selective tumor localization in experimental hepatoma by radiolabeled antiferritin antibody

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(83)90266-3· OSTI ID:5269580

Rostock, R A; Klein, J L; Leichner, P; Kopher, K A; Order, S E

The in vivo localization of /sup 131/I-radiolabeled antiferritin and normal IgG in the H-4-II-E rat hepatoma model was investigated by serial necropsy. Groups of 14 to 18 animals were injected with 500 ..mu..Ci (200 ..mu..g) of normal and antiferritin IgG. The total dose from the nonpenetrating beta radiation was calculated for tumor and normal tissue, and expressed as a targeting ratio of antiferritin to normal IgG for each organ studied. The results demonstrate 2.9 times greater dose deposition in tumors of animals treated with /sup 131/I-antiferritin than with /sup 131/I-normal IgG. /sup 131/I-antiferritin deposited equivalently in primary tumors and metastatic lesions of similar size. The specific binding in tumors could be competitively inhibited by the addition of unlabeled antiferritin but not unlabeled normal IgG. Specific targeting with /sup 131/I-antiferritin in comparison to /sup 131/I-normal IgG did not occur in any normal tissue. There was considerable variation in the dose deposition in different normal tissues.

• Research Organization: Johns Hopkins Hospital, Baltimore, MD
• OSTI ID: 5269580
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:9; ISSN IOBPD
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISEASES
DOSES
EXPERIMENTAL NEOPLASMS
FERRITIN
GLOBULINS
HEPATOMAS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
IRON COMPLEXES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALLOPROTEINS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIATION DOSES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RODENTS
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
VERTEBRATES