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Radiation enhancement of the efficiency of DNA-mediated gene transfer in CHO UV-sensitive mutants

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576747· OSTI ID:5269420
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We have employed the Chinese hamster ovary (CHO) UV-sensitive mutant cell lines, UV5 and UV20, to determine whether ionizing and ultraviolet irradiation enhance the efficiency of DNA-mediated gene transfer in cells deficient in excision repair. Confluent AA8 (wild type), UV5, and UV20 cells were transfected (via polybrene and dimethyl sulfoxide treatments) with the recombinant DNA plasmid, pSV2-gpt, trypsinized, irradiated with either X rays or ultraviolet in suspension, and then plated into flasks. After a 48-h expression time, cells were trypsinized, counted, and plated in XMAT media to select for pSV2-gpt transformation. We report that X-ray irradiation enhances gene transfer in wild-type AA8 and in both UV-sensitive cell lines. Ultraviolet irradiation enhances gene transfer in AA8 and UV20, but not in UV5. Since both UV20 and UV5 are deficient in excision repair, we suggest that ultraviolet-enhanced gene transfer may involve a postreplication repair mechanism deficient in UV5.
Research Organization:
B. C. Cancer Research Centre, Vancouver, British Columbia
OSTI ID:
5269420
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CHO CELLS
DNA
EFFICIENCY
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RECOMBINANT DNA
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
X RADIATION