Introduction of new genetic markers on human chromosomes

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90531-X· OSTI ID:5268838

Satoh, Hitoshi; Barrett, J C ^[1]; Oshimura, Mitsuo ^[2]

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1. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
2. Kanagawa Cancer Center Research Inst., Yokohama (Japan)

The purpose of this study was to use DNA transfection and microcell chromosome transfer techniques to engineer a human chromosome containing multiple biochemical markers for which selectable growth conditions exist. The starting chromosome was a t(X;3)(3pter{yields}3p12::Xq26{yields}Xpter) chromosome from a reciprocal translocation in the normal human fibroblast cell line GM0439. This chromosome was transferred to a HPRT (hypoxanthine phosphoribosyltransferase)-deficient mouse A9 cell line by microcell fusion and selected under growth conditions for the HPRT gene on the human t(X;3) chromosome. A resultant HAT-resistant cell line (A9(GM0439)-1) contained a single human t(X;3) chromosome. These results demonstrate that microcell chromosome transfer can be used to select chromosomes containing multiple markers.

• OSTI ID: 5268838
• Journal Information: Experimental Cell Research; (United States), Vol. 193:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HETEROCHROMOSOMES
GENE RECOMBINATION
HUMAN X CHROMOSOME
BIOLOGICAL MARKERS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
PLASMIDS
TUMOR CELLS
ANIMAL CELLS
CELL CONSTITUENTS
CHROMOSOMES
ENZYMES
GLYCOSYL TRANSFERASES
HUMAN CHROMOSOMES
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
TRANSFERASES
X CHROMOSOME
550400* - Genetics