Influence of nickel on the detection of nitrate reductase activity in sorghum extracts

Journal Article · Thu Jan 01 04:00:00 EST 1970 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.45.5.591· OSTI ID:5268017

Maranville, J W

The addition of nickel (4 x 10/sup -3/ M) to the extracting buffer enhances the nitrate reductase activity in preparations of young grain sorghum (Sorghum bicolor L.(Moench) leaf tissue by as much as 6-fold. Activities comparable to other plant species are obtained over an extraction pH range of 7 to 8 with tris buffer and reduced nicotinamide adenine dinucleotide as a cofactor for the reaction when the ratio of plant material to extraction medium is 1:20. The method also enhances nitrate reductase activity in sudangrass (Sorghum sudanense P. (Stapf)).

• Research Organization: Univ. of Nebraska, Lincoln
• OSTI ID: 5268017
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 45; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BEANS
BIOLOGICAL EFFECTS
CARBONIC ACID DERIVATIVES
CEREALS
CYANATES
CYANIDES
DATA
ELEMENTS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
FEDERAL REGION VII
FOOD
GRASS
INFORMATION
MAIZE
MANGANESE
METALS
NEBRASKA
NICKEL
NITROGEN COMPOUNDS
NORTH AMERICA
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
SORGHUM
TRANSITION ELEMENTS
USA
VEGETABLES
WHEAT