Regulation of adenosine 5'-phosphosulfate sulfotransferase activity by SO/sub 2/ in Rosa cells cultures
The effect of aeration with 5 ..mu..l l/sup -1/ SO/sub 2/ on extractable activity of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) activity from dark grown suspension cultures of Paul's Scarlet rose (Rosa sp.) was studied. The enzyme activity was at 50% of that of controls after 24 h and at 20% after 48 h. This decrease was only detected, when the pH of the nutrient solution was below 5.8, indicating that SO/sub 2/ rather than SO/sub 3//sup 2 -/ was the active species. The growth rate of the cells was not affected. After omission of SO/sub 2/ APSSTase activity increased to the level of control cultures within 24h. NO/sub 2/ up to 10 ..mu..ll/sup -1/ and Na/sub 2/SO/sub 3/ up to 500 ..mu..M had no effect on APSSTase activity. Addition of ascorbic acid to the culture medium did not affect the decrease in APSSTase activity induced by SO/sub 2/. Their results indicate that the effect of SO/sub 2/ on APSSTase activity is a specific regulatory phenomenon.
- Research Organization:
- Pflanzenphys Institut, Bern, Switzerland
- OSTI ID:
- 5268003
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENZYME ACTIVITY
INHIBITION
SULFUR DIOXIDE
BIOLOGICAL EFFECTS
BIOCHEMISTRY
CELL CULTURES
GROWTH
PH VALUE
CHALCOGENIDES
CHEMISTRY
OXIDES
OXYGEN COMPOUNDS
SULFUR COMPOUNDS
SULFUR OXIDES
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)