Correlation of 125I-LSD autoradiographic labeling with serotonin voltage clamp responses in Aplysia neurons

Journal Article · Wed May 01 00:00:00 EDT 1991 · Synapse (New York); (United States)

DOI:https://doi.org/10.1002/syn.890080104· OSTI ID:5266530

Evans, M L; Kadan, M J; Hartig, P R; Carpenter, D O ^[1]

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1. New York State Department of Health, State University of New York, Albany (USA)

Autoradiographic receptor binding studies using 125I-LSD (2-(125I)lysergic acid diethyamide) revealed intense labelling on the soma of a symmetrically located pair of cells in the abdominal ganglion of Aplysia californica. This binding was blocked by micromolar concentrations of serotonin and lower concentrations of the serotonergic antagonists, cyproheptadine and mianserin. Electrophysiological investigation of responses to serotonin of neurons in the left upper quadrant, where one of the labeled neurons is located, revealed a range of serotonin responses. Cells L3 and L6 have a K+ conductance increase in response to serotonin that is not blocked by cyproheptadine or mianserin. Cells L2 and L4 have a biphasic response to serotonin: a Na+ conductance increase, which can be blocked by cyproheptadine and mianserin, followed by a voltage dependent Ca2+ conductance which is blocked by Co2+ but not the serotonergic antagonists. Cell L1, and its symmetrical pair, R1, have in addition to the Na+ and Ca2+ responses observed in L2 and L4, a Cl- conductance increase blocked by LSD, cyproheptadine and mianserin. LSD had little effect on the other responses. The authors conclude that the symmetrically located cells L1 and R1 have a Cl- channel linked to a cyproheptadine- and mianserin-sensitive serotonin receptor that is selectively labelled by 125I-LSD. This receptor has many properties in common with the mammalian serotonin 1C receptor.

• OSTI ID: 5266530
• Journal Information: Synapse (New York); (United States), Journal Name: Synapse (New York); (United States) Vol. 8:1; ISSN 0887-4476; ISSN SYNAE
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIONS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CATIONS
CENTRAL NERVOUS SYSTEM AGENTS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
COBALT COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHYSIOLOGY
ESTERS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDOLES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPES
KINETICS
LABELLING
MEMBRANE PROTEINS
NERVE CELLS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOMIMETICS
PHYSIOLOGY
POTASSIUM COMPOUNDS
PROTEINS
PSYCHOTROPIC DRUGS
PYRROLES
QUATERNARY COMPOUNDS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RECEPTORS
SEROTONIN
SODIUM COMPOUNDS
SOMATIC CELLS
SYMPATHOMIMETICS
TRANSITION ELEMENT COMPOUNDS
TRYPTAMINES