Evidence for dual control mechanism regulating hepatic glucose output in nondiabetic men

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Diabetes; (United States)

DOI:https://doi.org/10.2337/diab.40.8.1033· OSTI ID:5266164

Clore, J N; Glickman, P S; Helm, S T; Nestler, J E; Blackard, W G ^[1]

---

1. Medical College of Virginia, Richmond (USA)

The authors previously reported a fall in hepatic glucose output (HGO) during sleep accompanied by reductions in glucose utilization (Rd) and free fatty acids (FFAs). This study was undertaken to determine the potential role of changes in Rd and FFA on HGO in nondiabetic men. To determine if the fall in HGO during sleep could be reversed by FFA elevation, seven nondiabetic men underwent (3-3H)glucose infusions from 2200 to 0800, with heparin (90 mU.kg-1.min-1) added at 0200. Glucose appearance (Ra) fell from 11.7 {plus minus} 1.1 at 2430 to 8.9 {plus minus} 0.8 mumol.kg-1.min-1 (P less than 0.05) at 0200. The fall in Ra was associated with decreases in FFA (0.57 {plus minus} 0.10 to 0.48 {plus minus} 0.07 mM) and glycerol (0.08 {plus minus} 0.01 to 0.06 {plus minus} 0.01 mM). Infusion of heparin significantly increased FFA and glycerol (1.09 {plus minus} 0.21 and 0.11 {plus minus} 0.01 mM, respectively, P less than 0.01) and resulted in a significant fall in plasma alanine, suggesting that gluconeogenesis had been increased. However, rates of glucose turnover were indistinguishable from overnight studies without heparin. In additional studies (n = 6), intralipid and heparin-induced FFA elevation (from 0.61 {plus minus} 0.07 to 0.95 {plus minus} 0.05 mM, P less than 0.01) stimulated gluconeogenesis ((U-14C)alanine to glucose) twofold (188 {plus minus} 22% increase compared to 114 {plus minus} 6% in saline control studies, P less than 0.01). However, despite increasing gluconeogenesis, overall HGO did not change (10.6 {plus minus} 0.5 vs. 10.7 {plus minus} 0.6 mumol.kg-1.min-1) during lipid infusion.

• OSTI ID: 5266164
• Journal Information: Diabetes; (United States), Vol. 40:8; ISSN 0012-1797
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
ALANINES
CARBON 14 COMPOUNDS
HEPARIN
HOMEOSTASIS
SLEEP
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
AMINES
AMINO ACIDS
ANTICOAGULANTS
CARBOHYDRATES
CARBON COMPOUNDS
CARBOXYLIC ACIDS
DRUGS
HEMATOLOGIC AGENTS
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
550501* - Metabolism- Tracer Techniques