Isolation and characterization of sulfite oxidase from Alligator mississipiensis

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5266015

Robbins, A; Neame, P J; Barber, M J ^[1]

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1. Univ. of South Florida College, Tampa (United States)

Sulfite oxidase has been isolated from fresh alligator liver using ammonium sulfate and acetone fractionation, DEAE chromatography and FPLC on Mono Q. The enzyme is dimeric and exhibits a subunit M. Wt. of approximately 58 kDa, larger than that of chicken SO. EPR spectroscopy of the partially-reduced enzyme revealed a single Mo(V) species while visible spectroscopy revealed the presence of cytochrome b{sub 557}. Maximal activities were obtained at pH 8 and 9, respectively. K{sub m}'s for SO{sub 3}{sup 2 {minus}}, cyt. c and Fe(CN){sub 6}{sup 3 {minus}} were 23.5 uM, 2.9 uM and 8.0 uM, respectively. Sequencing of peptides obtained by endoprotease K digestion indicated regions of extensive sequence similarity to chicken and rat enzymes in both heme and Mo-pterin domains. Regions of sequence dissimilarity were also found.

• OSTI ID: 5266015
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALLIGATORS
ANIMALS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
ELECTRON SPIN RESONANCE
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
MAGNETIC RESONANCE
METABOLISM
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
REPTILES
RESONANCE
SEPARATION PROCESSES
SPECTROSCOPY
SULFITES
SULFUR COMPOUNDS
VERTEBRATES