Growth and plating efficiency of methanococci on agar media. [Methanococcus maripaludis and M. voltae]

Journal Article · Fri Jul 01 00:00:00 EDT 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5266012

Jones, J W; Whitman, W B; Fields, R D; Wolfe, R S

Plating techniques for cultivation of methanogenic bacteria have been optimized for two members of the genus Methanococcus. Methanococcus maripaludis and Methanococcus voltae were cultivated on aerobically and anaerobically prepared agar media. Maintenance of O/sub 2/ levels below 5 mu l/liter within an anaerobic glove box was necessary during plating and incubation for 90% recovery of plated cells. Under an atmosphere of H/sub 2/, CO/sub 2/, and H/sub 2/S (79:20:1), 2 to 3 days of incubation at 37 degrees C were sufficient for the formation of visible colonies. The viability of plated cells was significantly affected by the growth phase of the culture, H/sub 2/S concentration, and the volume of medium per plate. In addition, colony size of methanococci was affected by agar type, as well as by the concentrations of agar, H/sub 2/S, and bicarbonate. 12 references

• Research Organization: Dept. of Microbiology, Univ., of Illinois, Urbana, IL 61801
• OSTI ID: 5266012
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 46:1
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
METHANOGENIC BACTERIA
BATCH CULTURE
AGAR
CARBON DIOXIDE
CULTURE MEDIA
HYDROGEN SULFIDES
OXYGEN
BACTERIA
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
COLLOIDS
DISPERSIONS
ELEMENTS
HYDROGEN COMPOUNDS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SULFIDES
SULFUR COMPOUNDS
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