Effects of hydrochloric acid on duodenal and jejunal mucosal permeability in the rat

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · American Journal of Physiology; (USA)

OSTI ID:5264799

Nylander, O; Kvietys, P; Granger, D N ^[1]

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1. Louisiana State Univ. Medical Center, Shreveport (USA)

The effects of various concentrations of hydrochloric acid (1, 5, 10, and 100 mM) on mucosal permeability and acid disappearance (H+-dis) in duodenum and jejunum were studied in anesthetized rats. Mucosal permeability was assessed by measuring blood-to-lumen clearance of 51Cr-labeled EDTA (ED-Cl). Luminal alkalinization (LA) and H+-dis were determined by backtitration. ED-Cl was stable during saline perfusion and was not affected by changes in intestinal blood flow. Basal ED-Cl was four times higher in duodenum than in jejunum. Mucosal permeability of both duodenum and jejunum was not altered by 1 mM HCl. However, 5 mM HCl induced a 3.3-fold increase (P less than 0.001) in ED-Cl in jejunum but was without effect in duodenum. A 15-fold increase in ED-Cl was obtained in jejunum and a doubling (P less than 0.001) in ED-Cl was observed in duodenum when HCl concentration was increased to 10 mM HCl. One hundred millimolar HCl induced large increases of ED-Cl in both segments. The twofold increase of ED-Cl in response to 10 mM HCl in duodenum was completely reversible, whereas ED-Cl in jejunum was three to four times higher (P less than 0.05) than preacid levels 60 min after cessation of acid perfusion. The net increase in jejunal ED-Cl obtained after acid exposure was closely correlated (r = 0.99) with the net increase in LA, indicating leakage of interstitial fluid into the luminal solution. LA (saline perfusion) and H+-dis (HCl perfusion) were significantly higher in duodenum than in jejunum.

• OSTI ID: 5264799
• Journal Information: American Journal of Physiology; (USA), Vol. 257; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDROCHLORIC ACID
BIOLOGICAL EFFECTS
MUCOUS MEMBRANES
PERMEABILITY
ADENOSINE
BLOOD FLOW
BLOOD PRESSURE
CHROMIUM 51
EDTA
ISOTOPE DILUTION
MUSCLES
RATS
SMALL INTESTINE
VASOPRESSIN
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HORMONES
HYDROGEN COMPOUNDS
INORGANIC ACIDS
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RIBOSIDES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques