Ischemia/reperfusion mediated oxygen free radical production in rat brain endothelium

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5264039

Grammas, P; Wood, K ^[1]; Liu, G J; Floyd, R A ^[2]; Wood, K ^[3]

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1. Univ. of Oklahoma, Oklahoma City (United States)
2. Oklahoma Medical Research Foundation, Oklahoma City (United States)
3. Univ. of Oklahoma Health Sciences Center, Oklahoma City (United States) Oklahoma Medical Research Foundation, Oklahoma City (United States)

Oxygen free radicals have been increasingly implicated in ischemia/reperfusion mediated injury to tissue. Recent methods of assessing tissue oxygen free radical flux including spin trapping, salicylate hydroxylation, protein oxidation and specific enzymatic activity loss have clearly shown that ischemia/reperfusion mediates oxidative damage in brain. Vascular endothelia cells are increasingly implicated in inactivating oxidative damage. The authors have used salicylate to assess hydroxyl free radical flux during an anoxia-reoxygenation insult in isolated brain microvessels. Brain microvessels that were subjected to a 20 min anoxia period and then reoxygenated for 20 min hydroxylated salicylate to form tissue localized 2,3-dihydroxybenzoic acid (2,3-DHBA) whereas microvessels that remained oxygenated throughout contained very little 2,3-DHBA. The data suggest that anoxia/reoxygenation of microvessels produces tissue localized hydroxyl free radical flux.

• OSTI ID: 5264039
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMAL TISSUES
ANIMALS
BIOSYNTHESIS
BODY
BRAIN
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
DISEASES
ENDOTHELIUM
HEMIC DISEASES
ISCHEMIA
MAMMALS
NERVOUS SYSTEM
ORGANS
PATHOGENESIS
RADICALS
RATS
RODENTS
SYMPTOMS
SYNTHESIS
TISSUES
VASCULAR DISEASES
VERTEBRATES