Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Proc. Amer. Phytopathol. Soc.; (United States)

OSTI ID:5263429

Lukezic, F L; Pell, E J; Levine, R G

Glycine max (L) Morrill, were grown in the greenhouse until the first trifoliate leaf was 1/3 expanded and exposed to 598 ..mu..g/m/sup 3/ (0.30 ppm) ozone for 3 or 4 h at 20/sup 0/C and 70% RH. Ozone at this concentration induced a low level of injury to primary leaves. At 24, 48, 96, 144 and 168 h after ozone exposure, bacteria were infiltrated into one primary leaf of a set of two plants/pot with the use of a vacuum desiccator. The opposite leaf and leaves of companion plants served as controls. Inoculum was a water suspension of 3 x 10/sup 7/ cells/ml of a Pseudomonas sp. that induced a hypersensitive response (HR). Inoculum at this concentration induced the typical rapid tissue collapse associated with HR. Plants were placed in plastic bags for 24 h after inoculation and maintained in the greenhouse. Ozone injury and HR were evaluated on the basis of surface area and intensity of symptoms. There were no significant differences in ozone injury levels in foliage when bacteria were subsequently infiltrated. Inhibition of HR was observed when leaves were inoculated 24 and 48 h after ozone exposure in each of 4 experiments. Inhibition of HR was observed when leaves were inoculated 24 and 48 h after ozone exposure in each of 4 experiments. Inhibition of HR was observed in 2 of 3 experiments 96 h after ozonation. Inhibition of HR was apparent in 1 of 2 experiments conducted 144 h after inoculation and not at all 168 h after ozone exposure.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 5263429
• Journal Information: Proc. Amer. Phytopathol. Soc.; (United States), Journal Name: Proc. Amer. Phytopathol. Soc.; (United States) Vol. 3; ISSN PAPSD
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
BACTERIA
DOSE-RESPONSE RELATIONSHIPS
GLYCINE HISPIDA
INFECTIVITY
INJURIES
LEAVES
LEGUMINOSAE
MICROORGANISMS
OZONE
PATHOGENS
PLANTS
POLLUTION
PSEUDOMONAS
SENSITIVITY
TOXICITY