Distribution of carbon flux within fatty acid utilization during myocardial ischemia and reperfusion

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Circulation Research (An Official Journal of the American Heart Association); (United States)

DOI:https://doi.org/10.1161/01.RES.69.3.779· OSTI ID:5263392

Nellis, S H; Liedtke, A J; Renstrom, B ^[1]

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1. Cardiovascular Research, University of Wisconsin Hospital and Clinics, Madison (USA)

Twenty-nine intact, working pig hearts were extracorporeally perfused and divided into two study groups (16 Aerobic and 13 Ischemic/Reflow hearts). Step function, equilibrium labeling with (14C)palmitate was used to develop uptake and washout curves of radioactive fatty acid products contained in coronary effluent during either aerobic perfusion or reperfusion after ischemia (60% reduction in left anterior descending coronary flow for 30 minutes). Left anterior descending control flows were slightly overperfused in Aerobic hearts (18% higher than in Ischemic/Reflow hearts); otherwise, circumflex and right coronary flows, left ventricular pressure, and serum fatty acids and blood sugar levels were comparable between groups. As expected in Ischemic/Reflow hearts, recovery of regional systolic shortening and myocardial oxygen consumption in reperfusion was only modestly impaired (-20% and -19%, respectively, not significant and p less than 0.011 compared with preischemic values, not significant from Aerobic hearts). The only significant metabolized product to be released from labeled fatty acid utilization in either group was 14CO2. A smaller fatty acid pool also was measured and accounted for by that contained in the coronary intravascular volume. The authors could determine no significant back diffusion of fatty acids from myocardium in either perfusion condition. Uptake time constants of the early phase of 14CO2 production also were virtually identical in both groups (19.9 {plus minus} 3.2 versus 16.7 {plus minus} 3.2 minutes in Aerobic and Ischemic/Reflow hearts, respectively) and strongly correlated with hemodynamics as described by heart rate. In washout studies, tissue radioactivity in the aqueous soluble and fatty acid pools declined in both study groups, and counts in complex lipids and cholesterol/cholesteryl esters remained steady, whereas those in triacylglycerols varied.

• OSTI ID: 5263392
• Journal Information: Circulation Research (An Official Journal of the American Heart Association); (United States), Journal Name: Circulation Research (An Official Journal of the American Heart Association); (United States) Vol. 69:3; ISSN CIRUA; ISSN 0009-7330
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANEMIAS
ANIMALS
BLOOD CIRCULATION
BLOOD PRESSURE
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
DISEASES
DOMESTIC ANIMALS
HEART
HEMIC DISEASES
IN VITRO
ISCHEMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOGENESIS
PERFUSED ORGANS
SWINE
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES